Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by TATEISHI, K.
Right arrow Articles by KITAGAWA, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by TATEISHI, K.
Right arrow Articles by KITAGAWA, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1976, Vol. 80, No. 1 191-194
© 1976 Japanese Biochemical Society

other

Enzyme Immunoassay of Testosterone Using the Testosterone-Glucoamylase Complex

Kayoko TATEISHI*, Hiroshi YAMAMOTO**, Toshio OGIWARA*, Chozo HAYASHI* and Masayasu KITAGAWA*

*Central Laboratory for Clinical Investigation, Osaka University Hospital Fukushima-ku, Osaka, Osaka 553
**Institute for Cancer Research, Osaka University Medical School Fukushima-ku, Osaka, Osaka 553

A highly sensitive, reproducible method was established for enzyme-coupled immunoassay of testosterone, involving coupling of testosterone to glucoamylase (EC 3.2.1.3 [EC] ) with a water-soluble coupling reagent carbodiimide. No enzyme activity was lost during this coupling procedure. The sensitivity of the method was comparable to that of competitive protein binding assay.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.