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J. Biochem, 1976, Vol. 80, No. 1 69-71
© 1976 Japanese Biochemical Society

research-article

Additional Evidence for Hydrophobic Bond Formation in the Adsorption of Sulfanilamide on Bio-Gel Beads

Masanobu JANADO, Kazuko SHIMADA, Naomi HORIE and Toshiro NISHIDA*

Biochemical Laboratory, Department of Food Science, Kyoto Women's University Kyoto 605, U.S.A.
*The Burnsides Research Laboratory, University of Illinois Urbana, Illinois 61801, U.S.A.

We have previously suggested the involvement of both hydrogen bonding and hydrophobic bonding in the adsorption of sulfanilamide on Bio-Gel beads. In the present study, we closely examined the concentration dependence of the binding curve and our proposed binding model has been corroborated. For comparison, binding parameters and thermodynamic data pertaining to the sulfanilamide-Sephadex system have been also evaluated.


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