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J. Biochem, 1977, Vol. 81, No. 4 1085-1090
© 1977 Japanese Biochemical Society

research-article

A Specific Method for the Determination of Threonine in Rat Blood Plasma Using Aldehyde Dehydrogenase

Teruo NISHIDA, Shingo KUME, Masayuki SAITO and Masami SUDA

Department of Medical Chemistry, Ehime University, School of Medicine Shigenobu, Onsen-gun, Ehime 791-02

A simple and sensitive method for the determination of threonine in rat blood plasma using aldehyde dehydrogenase after oxidation with periodate was developed. By the present method, threonine could be completely discriminated from serine and determined at the nanomole level. The amount of threonine in rat blood plasma obtained by the present method coincided well with the value determined on an amino acid analyzer.


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