J. Biochem, 1983, Vol. 94, No. 1 327-330
© 1983 Japanese Biochemical Society
other |
A New Method for Purification of Anti-Glycosphingolipid Antibody. Avian Anti-Hematoside (NeuGc) Antibody
*Department of Biochemistry, Shizuoka College of Pharmacy Oshika, Shizuoka, Shizuoka 422
**Department of Pathology, Research Institute for Microbial Diseases, Osaka University Suita, Osaka 565
A new method for purification of anti-glycosphingolipid antibodies has been developed. N-Glycolylneuraminyl(
2-3)lactosylceramide [hematoside (NeuGc)] could be hydrophobically bound on octyl-Sepharose 4B in the presence of 0.1M KCl. The Sepharose gel coated with hematoside (NeuGc) was used as immunoadsorbent for affinity column chromatography to purify avian anti-hematoside (NeuGc) antibody. The procedure is very simple, reproducible and applicable to purification of almost all anti-glycosphingolipid antibodies. The glycosphingolipid used for the affinity chromatography could be recovered without any destruction by successive extraction of the gel with methanol and methanol/chloroform (1 : 2, v/v).
1 Requests for reprints should be addressed to Dr. Makoto Matsumoto.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P. H. H. Lopez, G. Zhang, M. A. Bianchet, R. L. Schnaar, and K. A. Sheikh Structural requirements of anti-GD1a antibodies determine their target specificity Brain, July 1, 2008; 131(7): 1926 - 1939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Yuki, K. Susuki, M. Koga, Y. Nishimoto, M. Odaka, K. Hirata, K. Taguchi, T. Miyatake, K. Furukawa, T. Kobata, et al. Carbohydrate mimicry between human ganglioside GM1 and Campylobacter jejuni lipooligosaccharide causes Guillain-Barre syndrome PNAS, August 3, 2004; 101(31): 11404 - 11409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Alaniz, R. D. Lardone, S. L. Yudowski, M. I. Farace, and G. A. Nores Normally Occurring Human Anti-GM1 Immunoglobulin M Antibodies and the Immune Response to Bacteria Infect. Immun., April 1, 2004; 72(4): 2148 - 2151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Tangvoranuntakul, P. Gagneux, S. Diaz, M. Bardor, N. Varki, A. Varki, and E. Muchmore Human uptake and incorporation of an immunogenic nonhuman dietary sialic acid PNAS, October 14, 2003; 100(21): 12045 - 12050. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kaida, S. Kusunoki, K. Kamakura, K. Motoyoshi, and I. Kanazawa GalNAc-GD1a in human peripheral nerve: Target sites of anti-ganglioside antibody Neurology, August 26, 2003; 61(4): 465 - 470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Suzuki, T. Ito, T. Suzuki, R. E. Holland Jr., T. M. Chambers, M. Kiso, H. Ishida, and Y. Kawaoka Sialic Acid Species as a Determinant of the Host Range of Influenza A Viruses J. Virol., December 15, 2000; 74(24): 11825 - 11831. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ito, Y. Suzuki, T. Suzuki, A. Takada, T. Horimoto, K. Wells, H. Kida, K. Otsuki, M. Kiso, H. Ishida, et al. Recognition of N-Glycolylneuraminic Acid Linked to Galactose by the alpha 2,3 Linkage Is Associated with Intestinal Replication of Influenza A Virus in Ducks J. Virol., October 1, 2000; 74(19): 9300 - 9305. [Abstract] [Full Text]
|
|
|
|
|
|
C. W. Ang, B. C. Jacobs, A. H. Brandenburg, J. D. Laman, F. G. A. van der Meche, A. D. M. E. Osterhaus, and P. A. van Doorn Cross-reactive antibodies against GM2 and CMV-infected fibroblasts in Guillain-Barre syndrome Neurology, April 11, 2000; 54(7): 1453 - 1458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Buchwald, J. Bufler, M. Carpo, F. Heidenreich, R. Pitz, J. Dudel, E. Nobile-Orazio, and K.V. Toyka Combined pre- and postsynaptic action of IgG antibodies in Miller Fisher syndrome Neurology, January 9, 2000; 56(1): 67 - 74. [Abstract] [Full Text] [PDF]
|
|