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J. Biochem, 1984, Vol. 96, No. 4 1021-1026
© 1984 Japanese Biochemical Society

research-article

An Electron Spin Resonance Study of Temperature-Induced Structural Changes of the Spin-Labeled Myosin Head: An Evidence for Two Conformational States of Myosin Head1

Takenori YAMADA2, Yutaka KIRINO and Hiroshi SHIMIZU3

Faculty of Pharmaceutical Sciences, The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113

3To whom correspondence should be addressed

Heavy meromyosin labeled at the SH1 thiol group with an iodoacetamide spin label was studied by electron spin resonance spectroscopy at various temperatures in the presence and absence of nucleotides and PP1. The electron spin resonance spectra of the spin label bound to myosin head showed temperature-dependent changes indicating changes of the structure around the SH1thiol group of the myosin head. As the temperature was elevated, the bound spin label was more mobilized in all the systems examined. The mobilization of the bound spin label by the elevation of temperature was enhanced in the presence of nucleotides of PP1. The temperature-dependent spectral changes had isosbestic points indicating that the structural changes around the SH1 thiol group took place between two states of the bound spin label, a weakly immobilized and a strongly immobilized state.

1This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan

2Department of Physiology, School of Medicine, Teikyo University, ltabashi-ku, Tokyo 173


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