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J. Biochem, 1984, Vol. 96, No. 4 1109-1115
© 1984 Japanese Biochemical Society

research-article

Hybrid with Rabbit Skeletal DTNB-Light Chains of Heavy Meromyosin, Myosin, and Glycerinated Fibers from Akazara Scallop Adductor1

Takao OJIMA*, Kiyoyoshi NISHITA* and Shizuo WATANABE**

*Department of Chemistry, Faculty of Fisheries, Hokkaido University Hakodate, Hokkaido 041
**Department of Chemistry, Faculty of Technology, Gunma University Kiryu, Gunma 376

It was shown in our previous report (Ojima et al. (1983) J. Biochem. 94, 307–310) that hybridization of Akazara scallop "desensitized" myosin with rabbit skeletal DTNB-light chains led to inhibition of the Mg-ATPase activity of acto-desensitized myosin but to enhancement of its superprecipitation activity. The following are now found: (a) Development of tension in desensitized glycerinated fibers of Akazara adductor is significantly improved when DTNB-light chains are added to the fiber bath, (b) The actin-affinity of desensitized heavy meromyosin in the presence of ATP but in the absence of Ca2+ is decreased by hybridization with chicken gizzard 20K dalton-light chains but significantly increased by that with DTNB-light chains. It is therefore suggested that the increase in actin-binding may account for the enhancing effect of DTNB-light chains on the superprecipitation and on the tension development.

1This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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