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J. Biochem, 1984, Vol. 96, No. 4 1143-1153
© 1984 Japanese Biochemical Society

research-article

Predominant Synthesis of Fibroin Heavy and Light Chains on the Membrane-Bound Polysomes Prepared from the Posterior Silk Gland of the Silkworm, Bombyx mori1

Fumitaka OYAMA, Shigeki MIZUNO and Kensuke SHIMURA2

Laboratory of Biochemistry, Department of Agricultural Chemistry, Tohoku University, Tsutsumidori-Amamiyamachi Sendai, Miyagi1980

1. Membrane-bound polysomes were prepared from the posterior silk gland of the silkworm, Bombyx mori, on the fourth to fifth day in the fifth larval instar. The polysomes, when supplemented with a soluble fraction from the posterior silk gland, exhibited the elongation reaction of the growing polypeptide-chains, but the initiation reaction of polypeptide synthesis was not demonstrated in this system.

2. The predominant products synthesized on the membrane-bound polysomes were fibroin heavy chain (H-chain) and light chain (L-chain), while polypeptides of heterogeneous size classes were synthesized on the 105,000 × g-sedimentable polysomes.

3. A substantial fraction of the fibroin L-chain synthesized was bound to the H-chain by disulfide bond.

4. Most of the newly synthesized fibroin H- and L-chains on the membrane-bound polysomes were proved to be present within microsomal membrane vesicles because of their insensitivity to digestion with proteases in the absence of Triton X-100.

1This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.

2Present address: Laboratory of Biology, Tohoku Fukushi University, Kunimi, Sendai, Miyagi 980.


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