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J. Biochem, 1984, Vol. 96, No. 4 1193-1204
© 1984 Japanese Biochemical Society

research-article

Characterization of a Mini Plasmid Isolated from Shigella sonnei1

Shigeki SUGIURA*, Sakae NAKATANI*, Yukiko MIZUKAMI**, Tetsu HASE*, Hidco HIROKAWA** and Yukito MASAMUNE*

*Department of Microbiology, Faculty of Pharmaceutical Sciences, Kanazawa University Takaramachi 13–1, Kanazawa, Ishikawa 920
**Life Science Institute, Sophia University, 7–1 Kioi-cho Chiyoda-ku, Tokyo 102

A multicopy plasmid, 2.1kb in size, was isolated from Shigella sonnei and named pKYM. This plasmid is cryptic and isolated along with pKY-1, a ColE1-like plasmid. In this paper, we report the physical map of pKYM and some characters required for its multiplication. The replication of the plasmid DNA does not require DNA polymerase I but depends on protein(s) produced by itself. The plasmid is poorly mobilized by the F factor.

1Part of this work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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