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Journal of Biochemistry Advance Access published online on July 5, 2006
Journal of Biochemistry, doi:10.1093/jb/mvj141
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© 2006 The Japanese Biochemical Society.
Received April 13, 2006
Accepted May 24, 2006

Regular Paper

Lipids in Oxygen-Evolving Photosystem II Complexes of Cyanobacteria and Higher Plants

Isamu Sakurai 1, Jian-Ren Shen 2, Jing Leng 2, Shunsuke Ohashi 3, Masami Kobayashi 3, and Hajime Wada 1 *

1 Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo 153-8902, Japan
2 Department of Biology, Faculty of Science, Okayama University, Tsushima-naka, Okayama 700-8530/PRESTO, JST, Japan
3 Institute of Materials Science, University of Tsukuba, Ibaraki 305-8573, Japan

* To whom correspondence should be addressed.
Hajime Wada, E-mail: hwada{at}bio.c.u-tokyo.ac.jp


  Abstract

Lipids in dimeric photosystem II complexes prepared from two species of cyanobacteria, Thermosynechococcus vulcanus and Synechocystis sp. PCC6803, and two higher plants, spinach and rice, were analyzed to determine how many lipid molecules and what class of lipids are present in the photosystem II complexes. It was estimated that 27, 20, 8, and 7 lipid molecules per monomer are bound to the dimeric photosystem II complexes of T. vulcanus, Synechocystis, spinach, and rice, respectively. In each of the organisms, the lipid composition of the photosystem II complexes was quite different from that of the thylakoid membranes used for preparation of the complexes. The content of phosphatidylglycerol in the photosystem II complexes of each organism was much higher than that in the thylakoid membranes. Phospholipase A2 treatment of the photosystem II complexes of Synechocystis that degraded phosphatidylglycerol resulted in impairment of QB-mediated but not QA-mediated electron transport. These findings suggest that phosphatidylglycerol plays important roles in the electron transport at the QB-binding site in photosystem II complexes.

Keywords: Lipid; Phosphatidylglycerol; Photosynthesis; Photosystem II complex; Thylakoid membrane.
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