Skip Navigation



Journal of Biochemistry Advance Access published online on July 7, 2006
Journal of Biochemistry, doi:10.1093/jb/mvj145
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
140/2/247    most recent
mvj145v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Abe, E.
Right arrow Articles by Ito, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Abe, E.
Right arrow Articles by Ito, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2006 The Japanese Biochemical Society.
Received May 26, 2006
Accepted June 13, 2006

Regular Paper

A Novel Phosphatidylcholine Which Contains Pentadecanoic Acid at sn-1 and Docosahexaenoic Acid at sn-2 in Schizochytrium sp. F26-b

Eriko Abe 1, Yasuhiro Hayashi 1, Yoichiro Hama 2, Masahiro Hayashi 3, Masanori Inagaki 4, and Makoto Ito 1 *

1 Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenviromental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
2 Faculty of Agriculture, Saga University, 1 Honjyo, Saga 840-8502, Japan
3 Department of Biological Production and Enviromental Science, Faculty of Agriculture, The University of Miyazaki, Miyazaki 889-2192, Japan
4 Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan

* To whom correspondence should be addressed.
Makoto Ito, E-mail: makotoi{at}agr.kyushu-u.ac.jp


  Abstract

Docosahexaenoic acid (DHA, 22:6n-3)-containing phospholipids are a ubiquitous component of the central nervous system and retina, however their physiological and pharmacological functions have not been fully elucidated. Here, we report a novel DHA-containing phosphatidylcholine (PC) in a marine single cell eukaryote, Schizochytrium sp. F26-b. Interestingly, 31.8% of all the fatty acid in F26-b is DHA, which is incorporated into triacylglycerols and various phospholipids. In phospholipids, DHA was found to make up about 50% of total fatty acid. To identify phospholipid species containing DHA, the fraction of phospholipids from strain F26-b was subjected to normal phase high-performance liquid chromatography (HPLC). It was found that DHA was incorporated into PC, lyso-PC, phosphatidylethanolamine, and phosphatidylinositol. The major DHA-containing phospholipid was PC in which 32.5% of the fatty acid was DHA. The structure of PC was analyzed further by phospholipase A2 treatment, fast atom bombardment mass spectrometry, and 1H- and 13C-NMR after purification of the PC with reverse phase HPLC. Collectively, it was clarified that the major PC contains pentadecanoic acid (C15:0) at sn-1 and DHA at sn-2; the systematic name of this novel PC is therefore ‘1-pentadecanoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine’.

Keywords: DHA; odd-chain fatty acid; phosphatidylcholine; PC-DHA; phospholipid; Thraustochytrid.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.