Skip Navigation



Journal of Biochemistry Advance Access published online on September 23, 2006
Journal of Biochemistry, doi:10.1093/jb/mvj194
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
140/5/619    most recent
mvj194v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Akutagawa, A.
Right arrow Articles by Tamiya-Koizumi, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Akutagawa, A.
Right arrow Articles by Tamiya-Koizumi, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2006 The Japanese Biochemical Society.
Received June 22, 2006
Accepted September 5, 2006

Regular Paper

Disruption of Phospholipase C{delta}4 Gene Modulates the Liver Regeneration in Cooperation with Nuclear Protein Kinase C

Atsushi Akutagawa 1, Kiyoko Fukami 2, Yoshiko Banno 3, Tadaomi Takenawa 4, Reiji Kannagi 5, Yukihiro Yokoyama 6, Koji Oda 6, Masato Nagino 6, Yuji Nimura 6, Shonen Yoshida 7, and Keiko Tamiya-Koizumi 8 *

1 Division of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine, 65, Tsurumai-cho, Showa-ku, Nagoya 466 8550; Department of Molecular Pathology, Aichi Cancer Center Institute, 1-1, Kanokoden, Chikusa-ku, Nagoya 464-8681
2 Laboratory of Genome and Biosignal, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji-shi, Tokyo 192-0392
3 Department of Cell Signaling, Gifu University, Graduate School of Medicine, 1-1, Yanagido, Gifu 501-1194
4 Division of Biochemistry, Department of Cancer biology, Institute of Medical Science, University of Tokyo, 4-6-1, Shiroganedai, Minato-ku, Tokyo 108-8639
5 Department of Molecular Pathology, Aichi Cancer Center Institute, 1-1, Kanokoden, Chikusa-ku, Nagoya 464-8681
6 Division of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine, 65, Tsurumai-cho, Showa-ku, Nagoya 466 8550
7 Nagoya Kyoritsu Hospital, 1-172, Hokke, Nakagawa-ku, Nagoya 454-8525
8 Department of Molecular Pathology, Aichi Cancer Center Institute, 1-1, Kanokoden, Chikusa-ku, Nagoya 464-8681; Nagoya University School of Health Sciences, Nagoya University Graduate School of Medicine, 1-1-20, Daiko-minami, Higashi-ku, Nagoya 461-8673

* To whom correspondence should be addressed.
Keiko Tamiya-Koizumi, E-mail: kkoizumi{at}met.nagoya-u.ac.jp


  Abstract

Phospholipase C{delta}4 (PLC {delta}4) gene has been cloned from the cDNA library of regenerating rat liver. Using PLC {delta}4 gene-disrupted mice (PLC {delta}4-/-), we studied a role of PLC {delta}4 during liver regeneration after partial hepatectomy (PH). In PLC {delta}4-/-, liver regeneration occurred in an apparently normal way. However, BrdU-indices indicated that PLC {delta}4 gene disruption delayed the onset of DNA synthesis by 2 h. Noticeably, the BrdU- indices in PLC {delta}4+/+ remained rather constant throughout S phase, 25-35%, whereas in PLC {delta}4-/-, it fluctuated drastically from 25% at 34 h to 65% at late S, 42 h after PH. This fact showed that PLC {delta}4 gene disruption caused a higher synchronization of cell proliferation. The mRNA for PLC {delta}4 in PLC {delta}4+/+ appeared at late G1, and the expression continued throughout S phase. PLC activity increased transiently in chromatin at the late G1 and S phases in only PLC {delta}4+/+, but not in PLC {delta}4-/-. The specific increases in PLC activity well correlated with the transient increases of protein kinase C (PKC) a in chromatin of PLC {delta}4+/+. PKC {varepsilon} also increased transiently in chromatin from PLC {delta}4+/+ at late S. It is concluded that PLC {delta}4 regulates the liver regeneration in cooperation with nuclear PKC {alpha} and {varepsilon}.

Keywords: knockout mouse; liver regeneration; phospholipase C{delta}4; partial hepatectomy; protein kinase C.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.