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Journal of Biochemistry Advance Access published online on September 28, 2006
Journal of Biochemistry, doi:10.1093/jb/mvj196
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© 2006 The Japanese Biochemical Society.
Received July 11, 2006
Accepted September 13, 2006

Regular Paper

Regulation of the Homologous Two-Component Systems KvgAS and KvhAS in Klebsiella pneumoniae CG43

Ching-Ting Lin 1 and Hwei-Ling Peng 1 *

1 Department of Biological Science and Technology, National Chiao Tung University, Hsin Chu, Taiwan, Republic of China

* To whom correspondence should be addressed.
Hwei-Ling Peng, E-mail: hlpeng{at}cc.nctu.edu.tw


  Abstract

In Klebsiella pneumoniae CG43, deletion of the sensor gene kvgS reduced the kvgAS expression in M9 medium with 0.2 mM paraquat, 0.2 mM 2,2-dihydropyridyl, or 300 mM NaCl. This result shows an autoregulatory role of KvgS and a stress-responsive expression of the two-component system (2CS). The kvgS deletion also appeared to decrease the expression of kvhAS, paralogous genes of kvgAS. Additionally, measurements of the promoter activity in kvgA-mutant revealed that KvgA is probably an activator for the expression of kvgAS and kvhAS. The subsequent electrophoretic mobility shift assay, indicating a specific binding of the recombinant KvgA to the putative promoters PkvgAS and PkvhAS, also supported an interacting regulation between the 2CSs. In PkvgAS and PkvhAS, the presence of RpoS binding elements suggested an RpoS-dependent regulation. Nevertheless, the rpoS deletion reduced the expression of kvgAS but increased that of kvhAS. Moreover, the kvgA deletion reduced the expression of katG and sodC. The overexpression of KvhA altered the susceptibility to fosfomycin and an increasing activity of UDP-N-acetylglucosamine enolpyruvyl transferase, the target protein of fosfomycin, which suggesting a regulation by KvhA. Taken together, these indicated that the two 2CSs probably belong to different regulatory circuits of the RpoS regulon.

Keywords: two-component system; KvgAS; KvhAS; rpoS; katG; sodC; fosfomycin.
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