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Journal of Biochemistry Advance Access published online on October 18, 2006
Journal of Biochemistry, doi:10.1093/jb/mvj212
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© 2006 The Japanese Biochemical Society.
Received July 10, 2006
Accepted October 14, 2006

Regular Paper

Heat Shock Protein 40/DjB1 Is Required for Thermotolerance in Early Phase

Yukako Uchiyama 1, Naoki Takeda 2, Masataka Mori 3, and Kazutoyo Terada 1 *

1 Department of Molecular Genetics, Graduate School of Medical Sciences, Kumamoto University, Honjo 1-1-1, Kumamoto 860-8556
2 Division of Transgenic Technology, Center for Animal Resources and Development (CARD), Kumamoto University, Honjo 2-2-1, Kumamoto 860-0811
3 Department of Molecular Genetics, Graduate School of Medical Sciences, Kumamoto University, Honjo 1-1-1, Kumamoto 860-8556; Present address: Laboratory of Molecular Genetics, Faculty of Pharmaceutical Sciences, Sojo University, Ikeda 4-22-1, Kumamoto 860-0082

* To whom correspondence should be addressed.
Kazutoyo Terada, E-mail: terada{at}gpo.kumamoto-u.ac.jp


  Abstract

DjB1 (Hsp40/DnajB1/Hdj1) is a member of the Hsp40/DnaJ family that functions as a co-chaperone of mammalian Hsp70s. DjB1 recognizes substrate proteins and facilitates the ATPase activity of Hsp70. We generated DjB1 deficient mice. The DjB1-/- mice were viable and fertile with no obvious abnormalities, thus indicating that DjB1 is dispensable for development and viability. No difference was found between the DjB1-/- and wild-type peritoneal macrophages regarding resistance against various types of apoptosis-inducing reagents. However, DjB1-/- cells showed decreased thermotolerance in the early phase after mild heat treatment, but not in the late phase. After the heat treatment, Hsp70 was induced similarly in wild-type and DjB1-/- cells. Immunofluorescence staining of wild-type cells revealed the accumulation of DjB1 and Hsc70 in the nucleus after heat treatment. DjB1 also accumulated in the centrosome. The accumulation of Hsc70 in the nucleus was also observed in DjB1-/- cells. These results suggest that the impaired thermotolerance of DjB1-/- cells is not due to a mislocation of the Hsp70 family.

Keywords: DjB1 (Hsp40/DnajB1/Hdj1); Hsp70; Knockout mice; Molecular chaperone; Thermotolerance.
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