Skip Navigation



Journal of Biochemistry Advance Access published online on December 5, 2006
Journal of Biochemistry, doi:10.1093/jb/mvm006
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
141/1/93    most recent
mvm006v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Tsudzuki, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsudzuki, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2006 The Japanese Biochemical Society

Rat Brain Synaptic Vesicles are Devoid of Mg2+-ATPase Activity and Contain ß-Amyloid Precursor Protein

Toshihiro Tsudzuki

Department of Biochemistry I, School of Medicine, Nagoya City University, Kawasumi 1, Mizuho-ku, Nagoya 467-8601, Japan

Dr. Tsudzuki, Toshihiro, Kawasumi 1, Mizuho-cho, Mizuho-ku, Nagoya, Aichi, 467-8601, Tel: +81-52-853-8140, Fax: +81-52-841-3480, E-MAIL: tsudzuki{at}sunprom.med.nagoya-cu.ac.jp


  Abstract

Rat brain synaptic vesicles isolated by gel filtration on Sephacryl S-500 had little Mg2+(H+)-ATPase activity, though it was identified by Western blots with antibodies against the H+-ATPase A-subunit and other vesicle proteins. In contrast, tyrosine hydroxylase and dopa decarboxylase activities in the synaptic vesicles were substantial, suggesting that the absence of Mg2+(H+)-ATPase activity was not due to inactivation during isolation but rather to the nature of the synaptic vesicles. The vesicle component reactive to H+-ATPase antibody was also identified in the synaptosomal cytosol, so the antibody for the A-subunit seemed unnecessary to detect H+-ATPase. The synaptic vesicles contained ß-amyloid precursor protein of ~100 kDa. Based on these observations, synaptic vesicles without Mg2+(H+)-ATPase seemed to play a role(s) in the delivery of cytoplasmic and plasma membrane proteins to nerve terminals as well as in neurotransmission.

Key Words: H+-ATPase, ß-amyloid precursor protein, synaptic vesicles, dopa decarboxylase, tyrosine hydroxylase


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.