Skip Navigation



Journal of Biochemistry Advance Access published online on December 13, 2006
Journal of Biochemistry, doi:10.1093/jb/mvm014
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
141/1/1    most recent
mvm014v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Xu, X.
Right arrow Articles by Ito, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xu, X.
Right arrow Articles by Ito, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2006 The Japanese Biochemical Society

Fucosyl-GM1a, an endoglycoceramidase-resistant ganglioside of porcine brain

Xu Xua, Hatsumi Monjushoa, Masanori Inagakib, Yoichiro Hamac, Kuniko Yamaguchia, Keishi Sakaguchia, Masao Iwamorid, Nozomu Okinoa and Makoto Itoa,e,*

aDepartment of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan;
bGraduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan;
cFaculty of Agriculture, Saga University, 1 Honjo, Saga 840-8502, Japan;
dKinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan;
eBio-architecture Center, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan

*To whom correspondence should be addressed: Makoto Ito, Ph. D. Phone: 81-92-642-2901. Fax: 81-92-642-2907. E-mail: makotoi{at}agr.kyushu-u.ac.jp

Received July 5, 2006; Accepted September 25, 2006


  Abstract

The use of bovine brain has been prohibited in many countries because of the world-wide prevalence of mad cow disease, and thus porcine brain is expected to be a new source for the preparation of gangliosides. Here, we report the presence of a ganglioside in porcine brain which is strongly resistant to hydrolysis by endoglycoceramidase, an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. Five major gangliosides (designated PBG-1, 2, 3, 4, 5) were extracted from porcine brain by Folch's partition, followed by mild alkaline hydrolysis and PBA column chromatography. We found that PBG-2, but not the others, was strongly resistant to hydrolysis by the enzyme. After the purification of PBG-2 with Q-Sepharose, Silica gel 60 and Prosep-PB chromatographies, the structure of PBG-2 was determined by GC, GC-MS, FAB-MS and NMR spectroscopy as Fuc{alpha}1-2Galß1-3GalNAcß1-4(NeuAc{alpha}2-3)Galß1-4Glcß1-1’Cer (fucosyl- GM1a). The ceramide was mainly composed of C18:0 and C20:0 fatty acids and d18:1 and d20:1 sphingoid bases. The apparent kcat/Km for fucosyl-GM1a was found to be 30 times lower than that for GM1a, indicating that terminal fucosylation makes GM1a resistant to hydrolysis by the enzyme. This report indicates the usefulness of endoglycoceramidase to prepare fucosyl-GM1a from porcine brain.

Key Words: endoglycoceramidase, fucosyl-GM1a, ganglioside, glycosphingolipid, porcine brain


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GlycobiologyHome page
X. Xu, Y. Horibata, M. Inagaki, Y. Hama, K. Sakaguchi, H. M Goda, N. Okino, and M. Ito
A novel fucosyl glycosphingolipid of brine shrimp that is highly sensitive to endoglycoceramidase
Glycobiology, December 1, 2009; 19(12): 1446 - 1451.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.