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Journal of Biochemistry Advance Access published online on February 13, 2007
Journal of Biochemistry, doi:10.1093/jb/mvm047
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© 2007 The Japanese Biochemical Society

Identification of an inflammation-inducible serum protein recognized by anti-disialic acid antibodies as carbonic anhydrase II

Zenta Yasukawa§,{ddagger}, Chihiro Sato§,{dagger},* and Ken Kitajima§,{ddagger},{dagger}

From the {ddagger}Laboratory of Animal Cell Function, Bioscience and Biotechnology Center; the §Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences; and the {dagger}Institute for Advanced Research, Nagoya University, Nagoya 464-8601, JAPAN.

*Corresponding author: Chihiro Sato Bioscience and Biotechnology Center Nagoya University, Nagoya 464-8601, Japan Phone: +81-52-789-4297, Fax: +81-52-789-5228 E-mail: chi{at}agr.nagoya-u.ac.jp

Received January 17, 2007; Accepted January 20, 2007


  Abstract

Acute phase proteins are an important marker of inflammation and sometimes have a role in the general defense response towards tissue injury. In the present study, we identified a 32 kDa protein that was immunoreactive with monoclonal antibody 2-4B (mAb.2-4B), which is specific to di/oligoNeu5Gc structures, and that behaved as an acute phase protein following stimulation with either turpentine oil or lipopolysaccharides. The 32 kDa protein was identified as carbonic anhydrase II (CA-II), based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses of the purified protein. Mouse and human CA-II was immunoreactive and immunoprecipitated with mAb.2-4B, but contained no sialic acid. In addition to mAb.2-4B, the mAb. S2-566 an antibody specific for diNeu5Ac-containing glycans, recognized the CA-II, whereas an anti-oligo/polysialic acid antibody did not. These results indicate that a part of the CA-II protein structure mimics the disialic acid structure recognized by the monoclonal antibodies. This is the first report that CA-II circulates in the serum following inflammation.

Key Words: acute phase protein, carbonic anhydrase II, disialic acid, inflammation, serum


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