Skip Navigation



Journal of Biochemistry Advance Access published online on March 23, 2007
Journal of Biochemistry, doi:10.1093/jb/mvm077
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
141/5/729    most recent
mvm077v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Maeda, M.
Right arrow Articles by Kirita, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maeda, M.
Right arrow Articles by Kirita, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Japanese Biochemical Society

In vitro mineralization by mesenchymal stem cells cultured on titanium scaffolds

Masahiko Maeda1,2, Motohiro Hirose2,*, Hajime Ohgushi2 and Tadaaki Kirita1

1Department of Oral and Maxillofacial Surgery; Nara Medical University; 840 Shijo-cho; Kashihara, Nara 634-8521; Japan
2Research Institute for Cell Engineering (RICE); National Institute of Advanced Industrial Science and Technology (AIST); 3-11-46 Nakoji; Amagasaki, Hyogo 661-0974; Japan

* Corresponding author: Motohiro Hirose, Ph.D. Phone: +81-6-6494-7807, Fax: +81-6-6494-7861, E-mail: motohiro-hirose{at}aist.go.jp

Received December 3, 2006; Accepted February 27, 2007


  Abstract

Titanium has been utilized in the field of orthopedic and dental reconstructive surgery, but mineralization through osteogenic differentiation of osteogenic cells on titanium surfaces has not been fully investigated. Here we cultured rat mesenchymal stem cells (MSCs) on the surfaces of titanium dishes in osteogenic media containing calcein which is a calcium-binding fluorescence dye. On titanium dishes, MSCs showed high viability to adhere to the surfaces and excellent proliferation. At day 14 of culture, MSCs differentiated into osteoblasts to from mineralized matrices on titanium dishes as well as tissue culture polystyrene (TCPS) dishes which are widely recognized as optimal culture substrates. Calcein was incorporated into the bone minerals fabricated by MSCs cultured on both substrates to show green emission under fluorescence microscopy. The fluorescence intensity was quantified with an image analyzer during culture periods. These results indicate that the surfaces of titanium showed a high adhesion/proliferation potential to MSCs and that the titanium effectively supported the osteogenic differentiation of MSCs comparable to TCPS dishes. Therefore, the titanium is an effective scaffold that is applicable in bone reconstruction surgery.

Key Words: Calcein, Cell adhesion, Mesenchymal stem cell, Osteogenic differentiation, Titanium


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J R Soc InterfaceHome page
M Navarro, A Michiardi, O Castano, and J.A Planell
Biomaterials in orthopaedics
J R Soc Interface, October 6, 2008; 5(27): 1137 - 1158.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.