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Journal of Biochemistry Advance Access published online on April 24, 2007
Journal of Biochemistry, doi:10.1093/jb/mvm098
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© 2007 The Japanese Biochemical Society

Studying on the 19-bp palindrome repeats in human cytomegalovirus immediate early enhancer/promoter reveals their diversity in function for the promoter activity

Ping Shena,c, Gang Niua,c, Minghui Yaoa,c, Haoyue Wanga and Jian Feia,b,d,*

aModel Organism Research Center and Laboratory of Molecular Cell Biology/Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
bShanghai Nan Fang Model Organism Research Center, Pu Dong, Shanghai
cGraduate School of the Chinese Academy of Sciences
dModel Organism Division, E-Institutes of the Shanghai Universities,Shanghai Jiao Tong University

*Corresponding author: Jian Fei, Fax: +8621 58951005, E-mail: jfei{at}sibs.ac.cn

Received April 11, 2007; Accepted April 11, 2007


  Abstract

The human cytomegalovirus immediate early enhancer/promoter (HCMV MIEP), extending from -588 to +1 relative to the transcription start site, contains a series of reiterated cis-acting elements, such as 19-bp repeats, which occur four times in the enhancer/promoter region. However, it is still not clear whether these elements repeat just for backing up or they really execute various indispensable functions. We show here that these reiterated elements are functionally different from each other through serial deletion mutation and site-directed mutation. In addition, we also found that the CG-reverse in the first 19-bp repeat could improve the transcription activity of MIEP in Hela cells and impair the protein-binding capacity in the EMSA assay. The expression feature of this mutated MIEP in transgenic mice further confirmed its stronger and more universal transcription activity in vivo.

Key Words: HCMV, transcription regulation, MIEP, P sites, transgenic mice


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