Skip Navigation



Journal of Biochemistry Advance Access published online on June 1, 2007
Journal of Biochemistry, doi:10.1093/jb/mvm119
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
142/2/229    most recent
mvm119v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Oba, Y.
Right arrow Articles by Honjo, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oba, Y.
Right arrow Articles by Honjo, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Japanese Biochemical Society

Purification and characterization of tributyltin-binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus

Yumi Oba1, Yohei Shimasaki1,*, Yuji Oshima1, Hina Satone1, Takeshi Kitano2, Miki Nakao3, Shun-ichiro Kawabata4 and Tsuneo Honjo1

1 Laboratory of Marine Environmental Science, Division of Marine Biological Chemistry, Department of Bioscience and Biotechnology, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka, 812-8581, Japan
2 Department of Materials and Life Science, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan
3 Department of Bioscience and Biotechnology, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan
4 Department of Biology, Faculty of Sciences, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan

*Corresponding author: Yohei Shimasaki: Laboratory of Marine Environmental Science, Division of Marine Biological Chemistry, Department of Bioscience and Biotechnology, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka, 812-8581, Japan. Tel: +81-92-642-2906, Fax: +81-92-642-2908, E-mail: simasaki{at}agr.kyushu-u.ac.jp

Received May 9, 2007; Accepted May 24, 2007


  Abstract

We used gel filtration chromatography, anion-exchange chromatography, and polyacrylamide gel electrophoresis to purify tributyltin-binding protein type 2 (TBT-bp 2) from plasma of Japanese flounder (Paralichthys olivaceus) injected intraperitoneally with tributyltin (5.0 mg/kg body weight). Sodium dodecyl sulfate – polyacrylamide gel electrophoresis indicated that the molecular mass of TBT-bp 2 was approximately 48 kDa, and isoelectric focusing – polyacrylamide gel electrophoresis indicated that the isoelectric point was approximately 3.0. TBT-bp 2 contained 40% N-glycan. The complete cDNA nucleotide sequence and the genome sequence of TBT-bp 2 were determined by means of rapid amplification of cDNA ends of liver tissue of Japanese flounder and a genome-walking technique, respectively. The 216 amino acid sequence of TBT-bp 2 showed 47% identity to the sequences of puffer fish (Takifugu pardalis) saxitoxin- and tetrodotoxin-binding protein but only 27% similarity to the sequence of TBT-bp 1. Analysis of the motif sequence of the amino acid sequence and the structure of the gene encoding TBT-bp 2 suggested that this protein belongs to the lipocalin superfamily.

Key Words: detoxification, glycoprotein, Japanese flounder, lipocalin superfamily, serum protein


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.