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Journal of Biochemistry Advance Access published online on September 18, 2007
Journal of Biochemistry, doi:10.1093/jb/mvm176
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© 2007 The Japanese Biochemical Society

BAF53 interacts with p53 and functions in p53-mediated p21 gene transcription

Min Wang, Chunsheng Gu, Tianyang Qi, Wen Tang, Ling Wang, Shuyan Wang and Xianlu Zeng*

Institute of Genetics and Cytology, Northeast Normal University, Changchun, China

*To whom correspondence should be addressed: Dr. Xianlu Zeng, Tel: 86-431-85099317, Fax: 86-431-85681186, E-mail: zengx779{at}nenu.edu.cn

Received August 8, 2007; Accepted August 19, 2007


  Abstract

BAF53, a component of chromatin remodeling and histone acetyltransferase complexes, has been shown to be essential for cell survival in human cells and plays roles in p53-mediated gene transcription. However, the mechanism concerned in the process needs to be further explored. In this study, we show that BAF53 is involved in the repression of p53-dependent p21 gene transcription by interacting with p53 both in vivo and in vitro. Through EMSA and chromatin immunoprecipitation analyses, we demonstrate that BAF53 can reduce the p53 binding ability to p21 promoter. By western-blot experiments, we find that BAF53 can decrease p53-Lys382 acetylation, which may be partially responsible for the repression of p53 binding ability. Furthermore, BAF53 represses p21 promoter activity in a BRG1-independent manner. These data contribute to elucidating the molecular mechanisms of BAF53 in regulating p53-mediated gene transcription.

Key Words: BAF53, p53, p21 gene transcription, p53 acetylation


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