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Journal of Biochemistry Advance Access published online on November 16, 2007
Journal of Biochemistry, doi:10.1093/jb/mvm223
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© 2007 The Japanese Biochemical Society

Induction of cell adhesion by galectin-8 and its target molecules in Jurkat T cells

Hitomi Yamamoto1, Nozomu Nishi1,3, Hiroki Shoji1, Aiko Itoh3, Liang-Hao Lu1, Mitsuomi Hirashima2,3 and Takanori Nakamura1

1 Department of Endocrinology, and 2 Department of Immunology and Immunopathology, Faculty of Medicine, Kagawa University, Kagawa, Japan, and 3 Galpharma Co., Ltd., Kagawa, Japan.

Correspondence to: Prof. Takanori Nakamura, Ph. D. Department of Endocrinology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan. TEL: +81-87-891-2106, FAX: +81-87-891-2108, E-mail: tnaka{at}med.kagawa-u.ac.jp

Received September 3, 2007; Accepted November 6, 2007


  Abstract

We previously showed that tandem-repeat type galectin-8, which has two covalently linked carbohydrate recognition domains (CRDs), induces neutrophil-adhesion through binding to integrin {alpha}M. Here we analyzed the function of galectin-8 in Jurkat T-cells. Galectin-8, as well as tandem-repeat galectin-9, and several multivalent plant lectins, induced Jurkat T-cell adhesion to a culture plate, whereas single-CRD galectins-1 and -3 did not. Galectin-8 also induced the adhesion of peripheral blood leukocytes to HUVEC. These results suggest that the di- or multivalent structure of galectin-8 is essential for the induction of cell adhesion and that this ability exhibits broad specificity for leukocytes.

The galectin-8-induced cell adhesion was accompanied by stress fiber formation, which suggests that intracellular signaling is required. We have identified integrin {alpha}4 as one of the candidate target molecules associated with the induction of cell adhesion. Indeed, inhibition of the function of integrin {alpha}4 by treating cells with a blocking-antibody reduced the sensitivity to galectin-8. Also, the phosphorylation of Pyk and ERK1/2, indicators of integrin-mediated signaling, was up-regulated on treatment with galectin-8. Thus, a primary target of galectin-8 must be the sugar chains on members of the integrin family, which are abundantly expressed on the surface of leukocytic cells.

Key Words: adhesion, cytoskeleton arrangement, galectin-8, integrin, leukocyte


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