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Journal of Biochemistry Advance Access published online on June 11, 2008
Journal of Biochemistry, doi:10.1093/jb/mvn082
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© 2008 The Japanese Biochemical Society

Gene expression profiling of human mesenchymal stem cells for identification of novel markers in early- and late-stage cell culture

Shihori Tanabe§, Yoji Sato, Takayoshi Suzuki, Kazuhiro Suzuki, Taku Nagao and Teruhide Yamaguchi

Division of Cellular and Gene Therapy Products, National Institute of Health Sciences, Tokyo, 158-8501, Japan

§Corresponding author: Dr. Shihori Tanabe, Ph.D. Email address:stanabe{at}nihs.go.jp, Address: 1-18-1, Kami-yoga, Setagaya-ku, Tokyo, 158-8501, Japan, Phone: +81-3-3700-1141, Fax:+81-3-3700-9217

Received January 9, 2008; Accepted June 5, 2008


  Abstract

Summary

Human mesenchymal stem cells (hMSCs) are multipotent cells that differentiate into several cell types, and are expected to be a useful tool for cellular therapy. Although the hMSCs differentiate into osteogenic cells during early to middle stages, this differentiation capacity decreases during the late stages of cell culture.

To test a hypothesis that there are biomarkers indicating the differentiation potential of hMSCs, we performed microarray analyses and profiled the gene expression in 6 batches of hMSCs (passages 4–28). At least four genes (NDN, EPHA5, NOV, and RUNX2) were identified correlating with the passage numbers in all 6 batches.

The results showed that the osteogenic differentiation capacity of hMSCs is down-regulated in the late stages of cell culture. It seemed that adipogenic differentiation capacity was also down-regulated in late stage of the culture. The cells in late stage are oligopotent and the genes identified in this study have the potential to act as quality-control markers of the osteogenic differentiation capacity of hMSCs.

Key Words: cellular therapy, culture stage marker, differentiation, gene expression, stem cell


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