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Journal of Biochemistry Advance Access published online on August 11, 2008
Journal of Biochemistry, doi:10.1093/jb/mvn101
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© 2008 The Japanese Biochemical Society

Contribution of complement component C3 and complement receptor type 3 to carbohydrate-dependent uptake of oligomannose-coated liposomes by peritoneal macrophages

Yu Abe1, Yasuhiro Kuroda2, Noritaka Kuboki3, Misao Matsushita1, Naoaki Yokoyama3 and Naoya Kojima1,2

1Department of Applied Biochemistry, and 2Institute of Glycoscience, Tokai University, Hiratsuka, Kanagawa 259-1292, Japan; and 3National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

Correspondence to: Prof. Naoya Kojima. 1117 Kita-kaname, Hiratsuka-shi, Kanagawa 259-1292, Japan, TEL: 0463-58-1211 (Ex. 4645); FAX: 0463-50-2012, E-mail: naoyaki{at}keyaki.cc.u-tokai.ac.jp

Received July 17, 2008; Accepted August 7, 2008


  Abstract

Peritoneal macrophages (PEMs) preferentially and rapidly take up oligomannose-coated liposomes (OMLs) and subsequently mature to induce a Th-1 immune response following administration of OMLs into the peritoneal cavity. Here, we examine the contributions of complement component C3 and complement receptor type 3 (CR3) to carbohydrate-dependent uptake of OMLs by PEMs. Effective uptake of OMLs into PEMs in vitro was observed only in the presence of peritoneal fluid (PF), and OMLs incubated with PF were incorporated by PEMs in vitro in the absence of PF. These phenomena were inhibited by methyl-{alpha}-mannoside, N-acetylglucosamine or EDTA, but not by galactose. Pull-down analysis followed by peptide mass fingerprinting of PF-treated OMLs indicated that the OMLs were opsonized with complement fragment iC3b. In vivo uptake of OMLs by PEMs was inhibited by intraperitoneal injection of an antibody against CR3, a receptor for iC3b, and OML uptake by PEMs in the peritoneal cavity was not observed in C3-deficient mice. Thus, our results indicate that OMLs are opsonized with iC3b in a mannose-dependent manner in the peritoneal cavity and then incorporated into PEMs via CR3.

Key Words: complement C3, CR3, C-type lectin, macrophage, oligomannose


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Home page
 GlycobiologyHome page
H. Takagi, M. Numazaki, T. Kajiwara, Y. Abe, M. Ishii, C. Kato, and N. Kojima
Cooperation of specific ICAM-3 grabbing nonintegrin-related 1 (SIGNR1) and complement receptor type 3 (CR3) in the uptake of oligomannose-coated liposomes by macrophages
Glycobiology, March 1, 2009; 19(3): 258 - 266.
[Abstract] [Full Text] [PDF]


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