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Journal of Biochemistry Advance Access published online on October 6, 2008
Journal of Biochemistry, doi:10.1093/jb/mvn131
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© 2008 The Japanese Biochemical Society

Specific Protein Delivery to Target Cells by Antibody-Displaying Bionanocapsules

Naoya Kurata1, Takuya Shishido2, Masaru Muraoka2, Tsutomu Tanaka3, Chiaki Ogino4, Hideki Fukuda3 and Akihiko Kondo4

1Department of Chemical Science and Engineering, Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan
2Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan
3Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan
4Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan

Corespondence to: Prof. Akihiko Kondo. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan. Fax: +81 78 803 6196; Tel: +81 78 803 6196, E-mail: akondo{at}kobe-u.ac.jp

Received August 21, 2008; Accepted September 9, 2008


  Abstract

Bionanocapsules (BNCs) are nanoparticles with a high biocompatibility composed of the L protein of the hepatitis B virus surface antigen. BNC can deliver bioactive molecules to hepatocytes efficiently and specifically. However, delivery is limited to hepatocytes and incorporation of proteins into BNC is quite troublesome. Here, in order to alter the specificity of BNC and to achieve efficient protein delivery, we developed engineered BNC displaying the ZZ domain of protein A and incorporating EGFP inside the particles using an insect cell expression system. The ZZ domain displayed on the surface of BNC binds to anti-EGFR antibodies, allowing specific delivery of EGFP to HeLa cells. The engineered BNCs are a promising and powerful tool for efficient and cell specific protein delivery.

Key Words: protein delivery, bionanocapsule, insect cell expression system, ZZ domain, antibody-mediated targeting


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