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Journal of Biochemistry Advance Access published online on October 30, 2008
Journal of Biochemistry, doi:10.1093/jb/mvn143
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© The authors 2008. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

Trans-translation is involved in the CcpA-dependent tagging and degradation of TreP in Bacillus subtilis

Hiromi Ujiie1,4, Tomoko Matsutani1, Hisashi Tomatsu1,2, Ai Fujihara1,2, Chisato Ushida1- 4, Yasuhiko Miwa5, Yasutaro Fujita5, Hyouta Himeno1- 4 and Akira Muto1- 4,*

1Department of Biochemistry and Molecular Biology, Faculty of Agriculture and Life Science, and 2Department of Biology, Faculty of Science, 3RNA Research Center, Hirosaki University, Hirosaki 036-8561, Japan. 4The United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8551, Japan., 5Faculty of Biotechnology, Fukuyama University, Fukuyama 729-0292, Japan

*To whom correspondence should be addressed: Prof. Akira Muto, Tel: (81)172-39-3592; Fax: (81)172-39-3593; E-mail: muto@cc.hirosaki-u.ac.jp

Received September 30, 2008; Accepted October 17, 2008


  Abstract

TreP (trehalose-permease (phosphotransferase system (PTS) trehalose-specific enzyme IIBC component)) is one of the target proteins of tmRNA-mediated trans-translation in Bacillus subtilis [Fujihara et al. (2002) Detection of tmRNA-mediated trans-translation products in Bacillus subtilis. Genes Cells, 7, 343-350]. The TreP synthesis is subject to CcpA-dependent carbon catabolite repression (CCR), and the treP gene contains catabolite-responsive element (cre) sequence, a binding site of repressor protein CcpA, in the coding region. Here, we demonstrated that the tmRNA-tagging of TreP occurs depending on the gene for CcpA. In the presence of CcpA, the transcription of treP mRNA terminates at 8-9 nucleotides upstream of the 5’-edge of the internal cre sequence, and translational switch to the tag-sequence occurs at the 101st amino acid (asparagine) position from N-terminus of TreP. The results show that trans-translation reaction is involved in the tagging and degradation of the N-terminal TreP fragment produced by truncated mRNA, which is a product of transcriptional roadblock by CcpA binding to the cre sequence in the internal coding region.

Key Words: Bacillus subtilis, CcpA, tmRNA, trans-translation, TreP


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