Skip Navigation



Journal of Biochemistry Advance Access published online on March 24, 2009
Journal of Biochemistry, doi:10.1093/jb/mvp054
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
146/1/141    most recent
mvp054v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Chai, H. L.
Right arrow Articles by Miura, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chai, H. L.
Right arrow Articles by Miura, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

Accurate determination of carboxyl-terminal fragment of Presenilin 1 in various tissues from rat and cell lines

Hui Lin Chai and Satoshi Miura*

Radioisotope Research Center, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004

*To whom correspondence should be addressed: Radioisotope Research Center, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan. Tel.: +81-45-787-2760 Fax: +81-45-782-1251 E-mail: smiura{at}med.yokohama-cu.ac.jp.

Received March 16, 2009; Accepted March 16, 2009


  Abstract

Presenilin 1 (PS1) has been identified as a causative gene for the early-onset of familial Alzheimer's disease, and it is mainly localized in the endoplasmic reticulum and the Golgi membrane as a multiple membrane-spanning protein. In the cell, PS1 is proteolytically processed to a 30 kDa N-terminal fragment and a 20 kDa C-terminal fragment (CTF), both of which exist as a stable high molecular weight protein complex, together with other components of {gamma}-secretase. However, as there has been no report about the precise amount of PS1 expressed in mammalian tissues, the aim of this study was to quantitatively determine PS1-CTF amounts in various tissues such as liver, kidney, brain, and heart of rat by Western blotting using a [35S]-methionine-labeled PS1-CTF as a standard synthesized in a wheat germ cell-free protein synthesizing system. PS1-CTF contents in kidney, liver, brain, and heart were 17.0, 6.6, 6.4, and 0.2 fmol/mg protein, respectively. PS1-CTF contents were also determined in cultured cell lines such as HeLa, HEK293, and COS-1.

Key Words: Alzheimer's disease, Cell free protein synthesis, Presenilin 1, Quantitative Analysis, Western blotting


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.