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Journal of Biochemistry Advance Access published online on May 26, 2009
Journal of Biochemistry, doi:10.1093/jb/mvp079
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© The authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

Human C21orf63 is a heparin-binding protein

Kanae Mitsunaga1,4, Jun Harada Itadani2, Toshihide Shikanai1, Hiroaki Tateno3, Yuzuru Ikehara2, Jun Hirabayashi3, Hisashi Narimatsu1 and Takashi Angata1,5,6

From 1Glycogene Function Team, 2Molecular Medicine Team, and 3Lectin Application and Analysis Team, Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, JAPAN.

6To whom correspondence should be addressed: Dr. Angata, Takashi, Tel: +81-6879-8424 ext. 2480, E-mail: angata{at}sanken.osaka-u.ac.jp

Received March 3, 2009; Accepted May 18, 2009


  Abstract

Human C21orf63 is a type-1 transmembrane protein of hitherto unknown function, with two repeats of putative "galactose binding lectin domains". By using glycan microarray analysis and other assays, we found that human C21orf63 interacts with heparin and to a lesser extent with heparan sulfate. The C-terminal galactose binding lectin domain of C21orf63 is necessary for heparin binding. The inability of other human proteins with galactose binding lectin domains to interact with heparin suggests that heparin binding is a unique property of C21orf63. Results of real-time PCR and tissue immunostaining imply that C21orf63 is expressed on epithelia of various human tissues.

Key Words: C21orf63, glycan microarray, heparan sulfate, heparin, lectins

4Present address: Division of Stem Cell Regulation, Center for Molecular Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, JAPAN.

5Present address: the Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, JAPAN.


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