Skip Navigation



Journal of Biochemistry Advance Access published online on June 8, 2009
Journal of Biochemistry, doi:10.1093/jb/mvp087
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
146/3/399    most recent
mvp087v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Takahashi, S.
Right arrow Articles by Suiko, M.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takahashi, S.
Right arrow Articles by Suiko, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

Molecular Cloning, Expression, and Characterization of A Novel Mouse SULT6 Cytosolic Sulfotransferase

Saki Takahashi1,2, Yoichi Sakakibara*,1,2, Emi Mishiro1,2, Haruna Kouriki1, Rika Nobe1,2, Katsuhisa Kurogi1, Shin Yasuda3,4, Ming-Cheh Liu3 and Masahito Suiko1,2

1Department of Biochemistry and Applied Biosciences, University of Miyazaki, Miyazaki, Miyazaki, 889-2192 JAPAN
2United Graduate School of Agricultural Sciences, Kagoshima University,Kagoshima, Kagoshima, 890-0065 JAPAN
3Department of Pharmacology, College of Pharmacy, The University of Toledo, Toledo, OH 43606 USA
4Department of Bioscience, School of Agriculture, Tokai University, Aso, Kumamoto 869-1404, JAPAN

*To whom correspondence should be addressed: Dr. Yoichi Sakakibara: Department of Biochemistry and Applied Biosciences,University of Miyazaki,1-1, Gakuenkibanadai-Nishi, Miyazaki, Miyazaki 889-2192, JAPAN. Tel: 0985-58-7211, Fax: 0985-58-7211, Email: ysakaki{at}cc.miyazaki-u.ac.jp

Received March 31, 2009; Accepted May 19, 2009


  Abstract

By searching the mouse EST database, we identified a novel mouse cytosolic sulfotransferase (SULT) cDNA (RIKEN cDNA 2410078J06). Sequence analysis revealed that this new SULT belongs to the cytosolic SULT6 gene family. The recombinant form of this newly identified SULT, designated SULT6B1, was expressed using the pGEX-4T-1 glutathione S-transferase fusion system and purified from transformed BL21 Escherichia coli cells. Purified mouse SULT6B1 exhibited sulfonating activity toward thyroxine and bithionol among a variety of endogenous and xenobiotic compounds tested as substrates. pH optimum of purified mouse SULT6B1 was determined to be 8.0. Tissue-specific expression of mouse and human SULT6B1 was examined by RT-PCR. While human SULT6B1 was specifically expressed in kidney and testis, mouse SULT6B1 was detected in brain, heart, kidney, thymus, lung, liver and testis. Further studies are needed in order to clarify the role of SULT6B1 in the metabolism of thyroxine and possibly some xenobiotics in mouse.

Key Words: sulfotransferase, sulfonation, thyroxine, metabolism


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.