iTRAQ-based quantitative proteomic analysis of submandibular glands from rats with STZ-induced hyperglycemia
- Renato M.P. Alves1,
- Rui Vitorino1,
- Ana I. Padrão1,
- Daniel Moreira-Gonçalves2,
- José A. Duarte2,
- Rita M.P. Ferreira1 and
- Francisco Amado1,*
- 1QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal; and 2CIAFEL, Faculty of Sports, University of Porto, 4200-450 Porto, Portugal
- ↵*Francisco Amado, Escola Superior de Saúde da Universidade de Aveiro, Campus de Santiago, 3810-190 Aveiro, Portugal. Tel: +351-234-234-401-558, Fax: +351-234-401-597, email:
- Received July 26, 2012.
- Accepted October 23, 2012.
The impairment of salivary glands activity is often connected to the complaints of dry-mouth and subsequent degradation of the periodontium of diabetic patients. In this context, submandibular glands (SMGs) play a central role in saliva production and so the understanding of the molecular pathways affected is of paramount importance. Using a streptozotocin-induced hyperglycemia rat model and two different time points (2 and 4 months), we applied mass spectrometry-based proteomic techniques, validated with standard western blot analysis, to identify and quantify the effect of chronic hyperglycemia on the proteome of SMGs. We observed significant variations of proteins such as kallikreins, protein S100A6 or annexins. After 2 months of hyperglycemia, we observed an early phase response characterized by a significant increase of protein S100A6, linked to the inflammatory response, together with the impairment of metabolic and energy production processes. On the other hand, vesicular transport appeared to be favoured in such conditions. Interestingly, in a long-term response to hyperglycemia after 4 months of exposure, we observed a general attenuation of the variations. In conclusion, we present data that support the existence of an adaptation of the gland to long-term stress.
- isobaric tags for relative and absolute quantitation
- submandibular gland
- © The Authors 2012. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved